Confocal Microscopy

Confocal microscopy is able to simultaneously observe the interactions of biological objects with controlled surfaces and the structuring of these surfaces up to the sub-micron level.

 

Leader

Petithory Tatiana
Contact : tatiana.petithory@uha.fr

Description

Areas of activity :
Confocal microscopy observes and characterizes the interaction of biological objects with controlled surfaces, it also allows topographic characterization at micron and sub-micron level. This in particular leads on to studies in the cellular behaviour in the vicinity of physically or chemically structured surfaces.  

Main equipment (strengths of the Institute) :
The confocal microscopy platform is equipped with a Zeiss Axio Imager M2 LSM 700 confocal right microscope controlled by ZEN 2012 software.

Technical description

The confocal microscopy platform is equipped with a Zeiss Axio Imager M2 LSM 700 confocal right microscope controlled by ZEN 2012 software.

Motorized XY Piezo plate (D)(130* 85) • Piezo control
• Area of ​​use : 130mm x 35mm
• Maximum speed : 100 mm/s
• 0.2μm resolution
• Reproducibility +/- 1μm
• Absolute accuracy 1μm
Excitation lasers available 405nm diode, 488nm diode and 555nm diode
Filters • PMT1 : 420-480 nm, 420-550 nm
• PMT2 : LP420 nm, LP490 nm, LP640 nm, BP 510-550 nm, 420-550 nm
Objectives

Objectives

Digital introduction

Working distance
10x Air EC “Plan-Neofluar”

0.3

5.2 mm
20x Air “Apochromat Plan”

0.8

5.5 mm
20x Water W “Apochromat Plan” DIC

1

1.8 mm
50x Air EC “Epiplan-Neofluar”

0.8

0.58 mm
50x Air EC “Epiplan-Neofluar”

0.55

9.1 mm
63x Oil “Apochromat Plan”

1.4

0.19 mm
63x Water W “Apochromat Plan”

1

2.1 mm
Environment OkoLab incubation chamber, control of temperature and percentage of CO2
Data processing Computer allowing image analysis with ZEN 2012, IMARIS, Image J or ICY software.
Self-service accessibility yes – after training by the platform manager.