Confocal Microscopy
Confocal microscopy is able to simultaneously observe the interactions of biological objects with controlled surfaces and the structuring of these surfaces up to the sub-micron level.
Leader
Petithory Tatiana
Contact : tatiana.petithory@uha.fr
Description
Areas of activity :
Confocal microscopy observes and characterizes the interaction of biological objects with controlled surfaces, it also allows topographic characterization at micron and sub-micron level. This in particular leads on to studies in the cellular behaviour in the vicinity of physically or chemically structured surfaces.
Main equipment (strengths of the Institute) :
The confocal microscopy platform is equipped with a Zeiss Axio Imager M2 LSM 700 confocal right microscope controlled by ZEN 2012 software.

Technical description
The confocal microscopy platform is equipped with a Zeiss Axio Imager M2 LSM 700 confocal right microscope controlled by ZEN 2012 software.
Motorized XY Piezo plate (D)(130* 85) | • Piezo control • Area of use : 130mm x 35mm • Maximum speed : 100 mm/s • 0.2μm resolution • Reproducibility +/- 1μm • Absolute accuracy 1μm |
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Excitation lasers available | 405nm diode, 488nm diode and 555nm diode | ||||||||||||||||||||||||
Filters | • PMT1 : 420-480 nm, 420-550 nm • PMT2 : LP420 nm, LP490 nm, LP640 nm, BP 510-550 nm, 420-550 nm |
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Objectives
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Environment | OkoLab incubation chamber, control of temperature and percentage of CO2 | ||||||||||||||||||||||||
Data processing | Computer allowing image analysis with ZEN 2012, IMARIS, Image J or ICY software. | ||||||||||||||||||||||||
Self-service accessibility | yes – after training by the platform manager. |