Home » Confocal Microscopy

Confocal Microscopy

Confocal microscopy is able to simultaneously observe the interactions of biological objects with controlled surfaces and the structuring of these surfaces up to the sub-micron level.

 

Leader

Petithory Tatiana
Contact : tatiana.petithory@uha.fr

Description

Areas of activity :
Confocal microscopy observes and characterizes the interaction of biological objects with controlled surfaces, it also allows topographic characterization at micron and sub-micron level. This in particular leads on to studies in the cellular behaviour in the vicinity of physically or chemically structured surfaces.

Main equipment (strengths of the Institute) :

  • LSM 800 (Laser Scanning Microscope, ZEISS) Upright configuration equipped with Airyscan® super resolution module and reflexion module (topography)

  • SD Spinning Disk Confocal Microscope (Yokogawa, Nikon) inverted configuration

Technical description

  • LSM 800 (Laser Scanning Microscope, ZEISS) Upright configuration equipped with Airyscan® super resolution module and reflexion module (topography)

Statif

Axio imager M2 Upright

Motorized stage XY

• XY Piezo
• Range 130mm x 35 mm
• maximal speed : 100mm/s
• Accuracy 0,2μm
• reproducibility +/- 1μm•

LASER diodes

405nm, 488nm 555nm 640nm

Detectors

2 PMTs

Super Resolution

AiryScan detector GaAsp

Objectives
 

NA

WD

10xr EC « Plan-Neofluar »

0,3

5,2 mm

20x « Plan Apochromat »

0,8

5,5 mm

20x W « Plan Apochromat » DIC

1

1,8 mm

50x « Epiplan-Neofluar »

0,8

0,58 mm

50x LD EC « Epiplan-Neofluar »

0,55

9,1 mm

63x oil « Plan Apochromat »

1,4

0,19 mm

63x W « Plan Apochromat »

1

2,1 mm

Environnement

Incubation chamber OKOLAB T° CO2 and Humidity controller

Analyse

Software ZEN 2012, IMARIS, Image J ou ICY.

Accessibility

Technical training to use the system
  • SD Spinning Disk Confocal Microscope (Yokogawa, Nikon) inverted configuration

Statif

Eclipse Ti2- with  « perfect focus »

Motorised stage

10 nm res/300nm repetition en XY

0,2/0,4 nm/100nm repetition en Z (piezzo)

Light sources

LUMENCOR SOLA SMII :

wavelength 405nm ; 488nm ; 561nm ; 640nm

Objectives

–       10x oil, water, glycerol ON 0,5 CFI Plan Apochromat

–       20x water ON 0,95 CFI APO LWD (0,99-0,90) Lambda S

–       60x oil ON 1,4 CFI Plan APO LBDA

SD CSU-W1

–       Mono- disk (50µm)

–       Maximal speed 4000 rpm

–       Field of view 10x16 mm

Laser

–       Oxxius wave length 405-488-561-638 nm

Camera

–       sCMOS Hamamatsu Orca Flash 4.0

–       4,2 MegaPixel

–       Pixel size 6,2x6,2 µm

–       EQ  82 %

–       Air cooling

Incubation

Incubation chamber OKOLAB T° CO2 and Humidity controller

Software

–       NIS-Element